6BS9

Stage III sporulation protein AB (SpoIIIAB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-11-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.541
Spacegroup name	P 21 2 21
Unit cell lengths	74.540, 82.000, 84.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.000 - 2.320
R-factor	0.2093
Rwork	0.207
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.898
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.000	50.000	2.360
High resolution limit [Å]	2.320	6.290	2.320
Rmerge	0.131	0.063	0.602
Rmeas	0.136	0.065	0.644
Rpim	0.037	0.018	0.221
Number of reflections	23068	1274	1110
<I/σ(I)>	7.1
Completeness [%]	99.9	99.8	98.1
Redundancy	13.1	12.6	7.3
CC(1/2)		0.999	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	0.15 M potassium phosphate and 3.3 M ammonium sulfate