6BS5
Crystal structure of AMP-PNP-bound bacterial Get3-like A and B in Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.05969 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.129, 121.129, 120.542 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.282 - 3.100 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bs3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.321 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.282 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.243 | 0.958 |
| Number of reflections | 18969 | 2594 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG8000, calcium acetate |
