6BS4
Crystal structure of ATPgammaS-bound bacterial Get3-like A and B in Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.07822 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.629, 121.629, 120.058 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.230 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bs3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.230 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.124 | 0.802 |
| Number of reflections | 34876 | 3552 |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 97.0 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG8000, calcium acetate |
