6BS3
Crystal structure of ADP-bound bacterial Get3-like A and B in Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.07822 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.340, 121.340, 120.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.190 - 2.300 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.420 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.780 |
| Number of reflections | 51079 | 6691 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | PEG8000, calcium acetate |
