6BRT

F-box protein CTH with hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	183.843, 183.843, 153.662
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.158 - 2.393
R-factor	0.2483
Rwork	0.247
R-free	0.30600
RMSD bond length	0.009
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.158	2.479
High resolution limit [Å]	2.393	2.393
Number of reflections	115751
<I/σ(I)>	60.1
Completeness [%]	100.0
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.02 M amino acid mixture (Glu, Ala, Gly, Lys, and Ser); imidazole; 0.1 M MES monohydrate, pH 6.5, 40% glycerol, 20% PEG4000