6BRS

The Crystal Structure of the Ferredoxin Protease FusC in complex with Arabidopsis Ferredoxin, Ethylmercury phosphate soaked dataset

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	81.531, 127.369, 132.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.645 - 2.300
R-factor	0.1914
Rwork	0.189
R-free	0.24200
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.260	3.014
Rpim	0.049	0.788
Number of reflections	61936	4552
<I/σ(I)>	15.1	1.5
Completeness [%]	100.0	100
Redundancy	29.7	30.1
CC(1/2)	0.998	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M NaKPhos, 0.1 M Bistris Propane, 20% PEG3350