6BRS
The Crystal Structure of the Ferredoxin Protease FusC in complex with Arabidopsis Ferredoxin, Ethylmercury phosphate soaked dataset
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 81.531, 127.369, 132.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.645 - 2.300 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.260 | 3.014 |
| Rpim | 0.049 | 0.788 |
| Number of reflections | 61936 | 4552 |
| <I/σ(I)> | 15.1 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 29.7 | 30.1 |
| CC(1/2) | 0.998 | 0.595 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M NaKPhos, 0.1 M Bistris Propane, 20% PEG3350 |
