6BRQ

Crystal structure of rice ASK1-D3 ubiquitin ligase complex crystal form 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	298
Detector technology	CCD
Collection date	2017-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	77.908, 113.306, 92.858
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	91.710 - 2.990
Rwork	0.220
R-free	0.25600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.710	3.090
High resolution limit [Å]	2.990	2.990
Number of reflections	31045
<I/σ(I)>	26.4
Completeness [%]	96.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	150 mM Tris-HCL, pH 7.4, 22% MPD, 22% PEG1000, 22% P3350, 15 mM Sodium Citrate tribasic dihydrate pH 5.6, 0.45 M 1,6-Hexanediol, 5 mM DTT