6BRE
Hen Egg-White Lysozyme cocrystallized with Cadmium sulphate using CuKalpha source.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-10 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.410, 81.410, 36.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.740 - 1.860 |
| R-factor | 0.17419 |
| Rwork | 0.170 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.801 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.740 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.051 | 0.289 |
| Rmeas | 0.062 | 0.362 |
| Number of reflections | 18602 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 94.8 | 86 |
| Redundancy | 2.86 | 2.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 1.5 M Sodium Chloride, Sodium acetate |
