6BQ7
Crystal structure of Medicago truncatula Thermospermine Synthase (MtTSPS) in complex with spermidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.962, 79.962, 163.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.240 - 1.950 |
| R-factor | 0.1891 |
| Rwork | 0.188 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bq2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS (May 1, 2016) |
| Data scaling software | XDS (Nov 1, 2016) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.240 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.049 | 0.779 |
| Number of reflections | 39529 | 6238 |
| <I/σ(I)> | 207 | 2.23 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8.5 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 10% PEG3350, 0.1 M Magnesium Chloride, 0.1 M Bis-Tris propane buffer cryo 33% PEG400 |
