6BPO
The crystal structure of the Ferric-Catecholate import receptor Fiu from K12 E. coli: Closed form (P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.674, 133.159, 135.100 |
| Unit cell angles | 101.50, 107.42, 92.88 |
Refinement procedure
| Resolution | 29.230 - 2.900 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fp1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.310 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.222 | 1.199 |
| Rpim | 0.151 | 0.802 |
| Number of reflections | 116009 | 5714 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Bis-Tris Propane, 20 % PEG3350, 0.2 M Na-Malonate |
