6BOW
Human APE1 substrate complex with an T/T mismatch adjacent the THF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-18 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.259, 61.333, 73.119 |
| Unit cell angles | 83.31, 78.28, 86.96 |
Refinement procedure
| Resolution | 24.560 - 1.590 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.630 |
| High resolution limit [Å] | 1.591 | 1.600 |
| Rmerge | 0.063 | 0.508 |
| Number of reflections | 190771 | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 99.3 | 97.3 |
| Redundancy | 4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 7% PEG 20K, 100mM sodium Citrate, 15% glycerol, 5mM CaCl2 |
