6BO3
Structure Determination of A223, a turret protein in Sulfolobus turreted icosahedral virus, using an iterative hybrid approach
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.995, 56.995, 272.807 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.850 - 1.830 |
| R-factor | 0.16534 |
| Rwork | 0.164 |
| R-free | 0.19542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3j31 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.426 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.043 | 0.530 |
| Number of reflections | 47436 | 4662 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Crystals of A223-L208M were grown by hanging drop vapour diffusion using 2 micro-L of the protein solution and 2 micro-L in 0.1 M tri-sodium citrate dihydrate pH 5.6 and 35% t-butanol. |
