6BNX

Crystal structure of V278E-glyoxalase I mutant from Zea mays in space group P6(3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 63
Unit cell lengths	82.110, 82.110, 74.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.050 - 1.800
R-factor	0.1855
Rwork	0.183
R-free	0.22406
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d7z
RMSD bond length	0.014
RMSD bond angle	1.624
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.060	1.910
High resolution limit [Å]	1.800	1.800
Rmeas	0.067	1.375
Number of reflections	26692	4293
<I/σ(I)>	19.7	1.4
Completeness [%]	99.9	99.5
Redundancy	10.27	10.32
CC(1/2)	1.000	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.7	294	sodium formate 4.2 M pH 9.7, 0.5% PEG 4000