6BMD
RNA hairpin containing GpppG-oligo to mimic intermediate formed by activated monomer and activated downstream helper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2017-10-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.392, 63.759, 65.840 |
| Unit cell angles | 90.00, 122.53, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uz6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.190 | 0.377 |
| Rmeas | 0.213 | 0.429 |
| Rpim | 0.094 | 0.198 |
| Number of reflections | 7315 | 557 |
| <I/σ(I)> | 5.8 | 1.98 |
| Completeness [%] | 95.9 | 74.5 |
| Redundancy | 4.8 | 3.2 |
| CC(1/2) | 0.970 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM MgCl2, 3 M ammonia sulfate, 100 mM HEPES 7.5 |
