6BMA
The crystal structure of indole-3-glycerol phosphate synthase from Campylobacter jejuni subsp. jejuni NCTC 11168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.181, 148.181, 149.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.435 - 1.980 |
| R-factor | 0.1685 |
| Rwork | 0.167 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tsm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.450 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.075 | 0.667 |
| Rmeas | 0.084 | 0.765 |
| Rpim | 0.038 | 0.367 |
| Number of reflections | 43974 | 2128 |
| <I/σ(I)> | 29.3 | 2.5 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 4.8 | 4.2 |
| CC(1/2) | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 1.0M NaH2PO4/K2HPO4 |
