6BMA

The crystal structure of indole-3-glycerol phosphate synthase from Campylobacter jejuni subsp. jejuni NCTC 11168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97924
Spacegroup name	H 3 2
Unit cell lengths	148.181, 148.181, 149.947
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.435 - 1.980
R-factor	0.1685
Rwork	0.167
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tsm
RMSD bond length	0.006
RMSD bond angle	0.793
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.075	0.667
Rmeas	0.084	0.765
Rpim	0.038	0.367
Number of reflections	43974	2128
<I/σ(I)>	29.3	2.5
Completeness [%]	99.9	99.2
Redundancy	4.8	4.2
CC(1/2)		0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	1.0M NaH2PO4/K2HPO4