6BM4
Pol II elongation complex with an abasic lesion at i-1 position,soaking UMPNPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.720, 220.430, 193.150 |
| Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
| Resolution | 55.607 - 2.951 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.26010 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.300 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 126240 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 96.6 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 9-12% PEG6000 |
