6BM2
Pol II elongation complex with an abasic lesion at i-1 position
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.110, 222.270, 193.710 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 82.922 - 3.403 |
| R-factor | 0.2203 |
| Rwork | 0.219 |
| R-free | 0.24380 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.522 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.900 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 83778 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 9-12% PEG6000 |
