6BKR

Crystal structure of the A/Wyoming/3/2003 (H3N2) influenza virus hemagglutinin in complex with 6'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	H 3 2
Unit cell lengths	100.458, 100.458, 385.692
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.760
R-factor	0.16035
Rwork	0.159
R-free	0.18332
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o5n
RMSD bond length	0.011
RMSD bond angle	1.530
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Rpim	0.020	0.130
Number of reflections	74408
<I/σ(I)>	52	3.6
Completeness [%]	99.3	92.5
Redundancy	18	14.5
CC(1/2)	1.000	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1 M bicine pH 9 and 42% 2-methyl-2,4-pentanediol