6BK9
Crystal Structure of Squid Arrestin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 103.508, 103.508, 153.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.004 - 3.000 |
| R-factor | 0.294736677436 |
| Rwork | 0.292 |
| R-free | 0.34225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jsy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.000 |
| High resolution limit [Å] | 2.880 |
| Number of reflections | 11540 |
| <I/σ(I)> | 13.9 |
| Completeness [%] | 99.5 |
| Redundancy | 10.8 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.2 M Ammonium Sulphate, 300 mM KH2PO4, 100 mM Bis-Tris pH 6.5 |
