6BJQ

Eubacterium eligens beta-glucuronidase bound to glucuronic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	P 64 2 2
Unit cell lengths	179.788, 179.788, 134.494
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.965 - 2.700
R-factor	0.2192
Rwork	0.218
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.965	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.120	0.544
Rmeas	0.124	0.564
Rpim	0.033	0.149
Number of reflections	35651	4649
<I/σ(I)>	15.5	4.7
Completeness [%]	99.9	100
Redundancy	13.6	14.1
CC(1/2)	0.998	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	12% PEG 400, 0.1 M Bis-tris pH 6.5