6BJK
Human ABO(H) blood group glycosyltransferase GTB D302C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.490, 150.260, 78.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.580 - 2.120 |
| R-factor | 0.1953 |
| Rwork | 0.193 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lz7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.329 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.580 | 19.580 | 2.200 |
| High resolution limit [Å] | 2.120 | 4.550 | 2.120 |
| Rmerge | 0.088 | 0.053 | 0.294 |
| Rmeas | 0.088 | ||
| Number of reflections | 17135 | ||
| <I/σ(I)> | 9.1 | 21.2 | 3.9 |
| Completeness [%] | 95.3 | 87.5 | 98.8 |
| Redundancy | 4.68 | 4.87 | 4.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 277 | Spontaneous crystals were recovered from concentrated stocks of GTB/D302C (55 mg/mL) stored in 50 mM MOPS, pH 7.00, 0.1 M sodium chloride, 1 mM DTT, 5 mM manganese chloride, and kept at 277 K. |
