6BIT
SIRPalpha antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 164.966, 164.966, 96.716 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.622 - 2.191 |
| R-factor | 0.2118 |
| Rwork | 0.210 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.622 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 76976 | 6916 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.2 | 87.9 |
| Redundancy | 6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | Crystals were obtained by addition of 0.1 uL of protein to an equal volume of 22% PEG3350, 150 mM ammonium sulfate, and 0.1 M Tris (pH 7.0) |
