6BHX

B. subtilis SsbA with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	57.939, 93.681, 100.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.284 - 2.936
R-factor	0.2188
Rwork	0.216
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.487
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.920	2.920
Rmerge	0.128	0.496
Number of reflections	12270	597
<I/σ(I)>	14.48	2.29
Completeness [%]	99.7	98.4
Redundancy	7.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50% mixture with 50 mM MES pH 6.5, 7% PEG 8000, 100 mM magnesium acetate, 200 mM potassium chloride SsbA was incubated with a 1:2 SsbA to dT35 ratio and a-chymotrypsin prior to crystallization