6BGM
Crystal structure of ferrous form of the crosslinked human cysteine dioxygenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 131.757, 131.757, 34.262 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.939 - 2.206 |
R-factor | 0.183 |
Rwork | 0.179 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ic1 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
High resolution limit [Å] | 2.200 | 5.170 | 2.200 |
Rmerge | 0.185 | 0.114 | 0.839 |
Rmeas | 0.198 | 0.122 | 0.912 |
Rpim | 0.069 | 0.043 | 0.349 |
Number of reflections | 17488 | 1422 | 1345 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 8.1 | 8.1 | 6.6 |
CC(1/2) | 0.992 | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M MES, 2 M ammonium sulfate, 2% PEG400 |