6BGE
HELICOBACTER PYLORI ATPASE, HP0525, IN COMPLEX WITH 1G2 COMPOUND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 112.075, 112.075, 231.006 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.530 - 2.900 |
| R-factor | 0.22609 |
| Rwork | 0.223 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.440 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.080 | |
| Number of reflections | 18083 | 1737 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 92.4 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis Tris-HCl pH 6.5, 2.0 M ammonium sulfate. |
