6BG8

Shewanella frigidimarina ice-binding protein_1 DUF3494 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	101.2
Detector technology	CCD
Collection date	2016-05-27
Detector	RAYONIX MX300HE
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	44.288, 80.278, 72.736
Unit cell angles	90.00, 105.52, 90.00

Refinement procedure

Resolution	42.673 - 1.597
R-factor	0.185402565195
Rwork	0.184
R-free	0.21684
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Phyre2 Model
RMSD bond length	0.005
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.673
High resolution limit [Å]	1.597
Rmerge	0.177
Number of reflections	64562
<I/σ(I)>	5.94
Completeness [%]	99.1
Redundancy	3.8
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		277.15	2.4 M Ammonium Sulfate, 0.1 M citric acid (pH 5)