6BFI
Vinculin homolog in a sponge (phylum Porifera) reveals vertebrate-like cell adhesions involved in early multicellular evolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0059 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.344, 93.676, 101.634 |
| Unit cell angles | 114.81, 93.29, 90.23 |
Refinement procedure
| Resolution | 36.500 - 2.300 |
| R-factor | 0.2229 |
| Rwork | 0.218 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.451 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 74722 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 92.8 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M sodium / potassium tartrate, pH 8.0, 20% PEG3350 |
