6BDA
Wild-type I-OnuI bound to A3G substrate (post-cleavage complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.690, 68.980, 161.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.276 - 1.880 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.23260 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.810 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.980 |
| High resolution limit [Å] | 1.880 |
| Number of reflections | 39882 |
| <I/σ(I)> | 7.9 |
| Completeness [%] | 100.0 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Potassium Formate 20% W/V PEG3350 |
