6BD0
I-OnuI K227Y, D236A bound to cognate substrate (pre-cleavage complex)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.880, 66.740, 159.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.095 - 1.450 |
R-factor | 0.1659 |
Rwork | 0.165 |
R-free | 0.18990 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.796 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.740 | 1.460 |
High resolution limit [Å] | 1.450 | 1.450 |
Rpim | 0.046 | 0.334 |
Number of reflections | 80496 | |
<I/σ(I)> | 8.93 | |
Completeness [%] | 100.0 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.18 M Tri-ammonium Citrate 20% W/V PEG 3350 |