6BCT
I-LtrI E184D bound to non-cognate C4 substrate (pre-cleavage complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.611, 43.440, 105.210 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 33.547 - 2.730 |
| R-factor | 0.2296 |
| Rwork | 0.227 |
| R-free | 0.28160 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.690 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.220 | 2.860 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.106 | 0.590 |
| Number of reflections | 13720 | |
| <I/σ(I)> | 7.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M TRIS pH 7.0 20% W/V PEG 2000 MME |
