6BCF
I-LtrI G183A bound to cognate substrate (pre-cleavage complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.956, 66.770, 169.414 |
| Unit cell angles | 90.12, 90.08, 90.06 |
Refinement procedure
| Resolution | 38.992 - 2.920 |
| R-factor | 0.2425 |
| Rwork | 0.239 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r7p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.337 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.770 | 2.958 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.080 | 0.486 |
| Number of reflections | 72648 | |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 98.0 | 96.9 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris pH 7.0 20% W/V PEG2000 MME |
