6BC2
Cryo X-ray structure of acetylsisomicin bound AAC-VIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-05-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.409, 86.866, 50.313 |
| Unit cell angles | 90.00, 119.82, 90.00 |
Refinement procedure
| Resolution | 29.425 - 2.195 |
| R-factor | 0.283 |
| Rwork | 0.282 |
| R-free | 0.30760 |
| Starting model (for MR) | 6bc6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.940 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
| High resolution limit [Å] | 2.195 | 4.730 | 2.200 |
| Rmerge | 0.094 | 0.050 | 0.554 |
| Number of reflections | 14753 | 1378 | 1572 |
| <I/σ(I)> | 16 | ||
| Completeness [%] | 91.8 | 83.9 | 97.3 |
| Redundancy | 3 | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 285 | 0.1 M Tris, pH 8.5, 0.3 M magnesium chloride, 15-20% PEG8000 |
