6BBO

Crystal structure of human APOBEC3H/RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 31 1 2
Unit cell lengths	101.293, 101.293, 211.217
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.250 - 3.428
R-factor	0.3652
Rwork	0.364
R-free	0.39210
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6b0b
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2926)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	3.780
High resolution limit [Å]	3.428	3.450
Rmerge	0.188	1.554
Rmeas	0.241
Number of reflections	16797	3988
<I/σ(I)>	5.5	1.1
Completeness [%]	99.9	99.9
Redundancy	4.7	4.6
CC(1/2)		0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NaI, PEG3350