6BAT

Crystal Structure of Wild-Type GltPh in complex with L-aspartate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953700
Spacegroup name	P 61
Unit cell lengths	114.729, 114.729, 322.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.400
R-factor	0.2492
Rwork	0.247
R-free	0.28650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nwx
RMSD bond length	0.008
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.076	0.770
Rmeas	0.103
Rpim	0.069	0.706
Number of reflections	32029	13872
<I/σ(I)>	5.8	0.9
Completeness [%]	97.2	99.1
Redundancy	3	2.9
CC(1/2)	0.998	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	50 mM citric acid 50 mM Disodium phosphate 100 mM Lithium sulfate 15-32 % PEG1000