6BAS
Crystal structure of Thermus thermophilus Rod shape determining protein RodA D255A mutant (Q5SIX3_THET8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 70 |
| Detector technology | PIXEL |
| Collection date | 2017-08-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0333 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.405, 80.012, 47.827 |
| Unit cell angles | 90.00, 91.06, 90.00 |
Refinement procedure
| Resolution | 40.006 - 3.194 |
| R-factor | 0.2793 |
| Rwork | 0.277 |
| R-free | 0.30240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bar |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.751 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2880: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.006 | 3.380 |
| High resolution limit [Å] | 3.194 | 3.200 |
| Number of reflections | 13756 | 1200 |
| <I/σ(I)> | 4.08 | 0.44 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.43 | 3.35 |
| CC(1/2) | 0.996 | 0.417 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | Reconstituted in 10:1 monoolein:cholesterol mix. Precipitant solution: 35-50% PEG 200, 100 mM NaCl, 100mM MgCl2, 0.1 M Tris pH 7.6-8.3 |
