6BAN

Potent and Selective Antitumor Activity of a T-Cell Engaging Bispecific Antibody Targeting a Membrane-Proximal Epitope of ROR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	86.790, 84.360, 102.740
Unit cell angles	90.00, 113.88, 90.00

Refinement procedure

Resolution	41.040 - 1.950
R-factor	0.162
Rwork	0.160
R-free	0.19600
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BALBES (2ZNW)
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.770	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.081	0.335
Rmeas	0.096	0.396
Rpim	0.069	0.208
Number of reflections	98668	4904
<I/σ(I)>	11.4	4.1
Completeness [%]	99.8	100
Redundancy	3.5	3.6
CC(1/2)	0.990	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M Li Sulfate, 20% PEG3350