Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.15 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 234.282, 253.842, 73.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.140 - 2.900 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e9o 1rz7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 172.163 | 117.141 | 3.060 |
| High resolution limit [Å] | 2.905 | 9.190 | 2.900 |
| Rmerge | 0.048 | 1.433 | |
| Rmeas | 0.179 | 0.053 | 1.559 |
| Rpim | 0.071 | 0.021 | 0.607 |
| Total number of observations | 301272 | 9954 | 45454 |
| Number of reflections | 48731 | 1682 | 7089 |
| <I/σ(I)> | 8.3 | 24.3 | 1.4 |
| Completeness [%] | 99.4 | 99 | 99.8 |
| Redundancy | 6.2 | 5.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 20% PEG 3350, 200mM sodium malonate |
