6B94

Crystal structure of Human galectin-1 in complex with Lactulose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	43.230, 58.320, 111.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.730 - 1.800
R-factor	0.21042
Rwork	0.209
R-free	0.23343
RMSD bond length	0.009
RMSD bond angle	1.477
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.730
High resolution limit [Å]	1.800	1.800
Number of reflections	26735
<I/σ(I)>	20.9
Completeness [%]	99.7
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium sulfate, 25% w/v PEG4000, 0.1 M sodium acetate trihydrate, pH 6.2