6B91
Crystal structure of the N-terminal domain of human METTL16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-25 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 190.050, 190.050, 190.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.510 - 1.940 |
R-factor | 0.182 |
Rwork | 0.181 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h00 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.624 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.067 | 1.370 |
Rmeas | 0.069 | 1.420 |
Number of reflections | 43441 | 6791 |
<I/σ(I)> | 25 | 2 |
Completeness [%] | 99.7 | 98.2 |
Redundancy | 16.2 | 14.2 |
CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.3 M K2HPO4, 45 mM NaH2PO4; drop contained 4 uL of protein solution (27 mg/mL) and 2 uL of reservoir solution, 25% ethylene glycol was used for cryo-protection |