6B8R
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-10-11 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.175, 60.262, 38.423 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 38.320 - 1.650 |
| R-factor | 0.1853 |
| Rwork | 0.184 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.827 |
| Data reduction software | SAINT (v8.18c) |
| Data scaling software | XPREP (2008/2 for Windows) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.028 | 0.281 |
| Number of reflections | 17112 | 303 |
| <I/σ(I)> | 23.12 | |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 5.56 | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
