6B8N
Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-08-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 108.299, 143.382, 164.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.977 - 2.201 |
R-factor | 0.2214 |
Rwork | 0.220 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6b8l |
RMSD bond length | 0.002 |
RMSD bond angle | 0.419 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 14.977 | 14.977 | 2.330 |
High resolution limit [Å] | 2.200 | 6.100 | 2.200 |
Rmerge | 0.164 | 0.034 | 2.345 |
Rmeas | 0.181 | 0.038 | 2.599 |
Total number of observations | 689772 | ||
Number of reflections | 124961 | 5504 | 19779 |
<I/σ(I)> | 8.32 | 39.83 | 0.72 |
Completeness [%] | 99.6 | 93.2 | 99.5 |
Redundancy | 5.52 | 5.581 | 5.419 |
CC(1/2) | 0.997 | 0.999 | 0.424 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 2M ammonium sulfate, 0.1M BisTris pH 6.5, 0.01mM CaCl2 |