6B8N

Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-08-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	108.299, 143.382, 164.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.977 - 2.201
R-factor	0.2214
Rwork	0.220
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b8l
RMSD bond length	0.002
RMSD bond angle	0.419
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	14.977	14.977	2.330
High resolution limit [Å]	2.200	6.100	2.200
Rmerge	0.164	0.034	2.345
Rmeas	0.181	0.038	2.599
Total number of observations	689772
Number of reflections	124961	5504	19779
<I/σ(I)>	8.32	39.83	0.72
Completeness [%]	99.6	93.2	99.5
Redundancy	5.52	5.581	5.419
CC(1/2)	0.997	0.999	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	2M ammonium sulfate, 0.1M BisTris pH 6.5, 0.01mM CaCl2