6B8L

Crystal Structure of the Apo/CaM:Kv7.4 (KCNQ4) AB Domain Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-03-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9797
Spacegroup name	I 2 2 2
Unit cell lengths	108.435, 142.453, 164.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.888 - 2.300
R-factor	0.2031
Rwork	0.198
R-free	0.24560
RMSD bond length	0.007
RMSD bond angle	0.840
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.119	0.046	0.928
Rmeas	0.128	0.049	0.998
Rpim	0.047	0.019	0.366
Number of reflections	57501	3056	2833
<I/σ(I)>	13.6
Completeness [%]	100.0	99.6	100
Redundancy	7.3	7.1	7.4
CC(1/2)		0.999	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	2M ammonium sulfate, 0.1M BisTris pH 6.5
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	2M ammonium sulfate, 0.1M BisTris pH 6.5