6B87

Crystal structure of transmembrane protein TMHC2_E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	103.485, 121.602, 51.952
Unit cell angles	90.00, 119.85, 90.00

Refinement procedure

Resolution	36.942 - 2.947
R-factor	0.2595
Rwork	0.258
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.257
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.950	2.950
Number of reflections	10899
<I/σ(I)>	9.6
Completeness [%]	92.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05 M magnesium acetate tetrahydrate, 0.05 M sodium acetate, pH 5.4, 30% v/v PEG400