6B85

Crystal structure of transmembrane protein TMHC4_R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	P 4
Unit cell lengths	80.240, 80.240, 251.572
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.992 - 3.889
R-factor	0.294
Rwork	0.291
R-free	0.32240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.558
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.900	3.900
Number of reflections	14545
<I/σ(I)>	18.2
Completeness [%]	99.6
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	30% v/v PEG400, 100 mM MOPS, pH 7.0, 100 mM sodium chloride, 10 mM DDAO