6B7V

Structure of hen egg-white lysozyme pre-treated with high-pressure homogenization at 120 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.459
Spacegroup name	P 43 21 2
Unit cell lengths	78.144, 78.144, 36.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.440 - 1.482
R-factor	0.1694
Rwork	0.168
R-free	0.20330
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.014
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	34.950
High resolution limit [Å]	1.480
Rmerge	0.047
Rmeas	0.048
Number of reflections	19194
<I/σ(I)>	41.89
Completeness [%]	98.0
Redundancy	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.7M sodium chloride, 0.1M sodium acetate pH 4.8