6B7T
Truncated strand 10-less green fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.369, 67.212, 59.991 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 35.123 - 1.910 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.120 | 35.120 | 1.720 |
| High resolution limit [Å] | 1.690 | 9.080 | 1.690 |
| Rmerge | 0.060 | 0.028 | 2.105 |
| Rmeas | 0.071 | 0.034 | 2.651 |
| Rpim | 0.037 | 0.017 | 1.574 |
| Total number of observations | 152607 | 1073 | 3659 |
| Number of reflections | 42227 | 295 | 1429 |
| <I/σ(I)> | 10.2 | 39.6 | 0.5 |
| Completeness [%] | 96.4 | 97.8 | 63.9 |
| Redundancy | 3.6 | 3.6 | 2.6 |
| CC(1/2) | 0.999 | 0.999 | 0.189 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M HEPES pH 7.0, 0.2M ammonium chloride, 22.5 v/v% PEG 6000 |
