6B7R

Truncated strand 11-less green fluorescent protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.22
Spacegroup name	P 43
Unit cell lengths	55.548, 55.548, 139.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.602 - 1.730
R-factor	0.1592
Rwork	0.158
R-free	0.18390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.790
High resolution limit [Å]	1.730	3.730	1.730
Rmerge	0.098	0.047	1.053
Rmeas	0.106	0.050	1.139
Rpim	0.039	0.018	0.432
Number of reflections	44426	4493	4416
<I/σ(I)>	10
Completeness [%]	100.0	99.8	100
Redundancy	7.3	7.3	6.9
CC(1/2)		0.998	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M CHES pH 9.5, 1.0M trisodium citrate