6B7P
Crystal structure of Legionella effector sdeD (lpg2509)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | H 3 |
| Unit cell lengths | 154.349, 154.349, 89.568 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.000 - 1.510 |
| R-factor | 0.16903 |
| Rwork | 0.168 |
| R-free | 0.19448 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.174 |
| High resolution limit [Å] | 1.510 |
| Number of reflections | 124885 |
| <I/σ(I)> | 29.2 |
| Completeness [%] | 99.5 |
| Redundancy | 5.083 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | PEG3350 12%, 0.1M MES pH5.2, 0.2M NH4CL |
