6B72
A novel HIV-1 Nef dimer interface induced by a single octyl-glucoside molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 109.577, 109.577, 247.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.900 - 3.200 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.900 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.075 | 0.828 |
| Rmeas | 0.079 | 0.869 |
| Rpim | 0.024 | 0.264 |
| Number of reflections | 29203 | 4209 |
| <I/σ(I)> | 20.5 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 18%-20% PEG5000 MME, 0.1 M Bis-Tris propane, pH 8.0, 5% glycerol |
