6B6N
Orthorhombic trypsin (295 K) in the presence of 50% mpd
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION NOVA |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2017-03-02 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.692, 58.538, 67.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.169 - 2.000 |
R-factor | 0.1225 |
Rwork | 0.121 |
R-free | 0.15660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.520 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.200 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 15002 | 2175 |
<I/σ(I)> | 17.1 | 7.4 |
Completeness [%] | 99.0 | 99.1 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 1.000 | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Well: 25% P8K, 0.2 M AmSO4, 0.1 M benzamidine Hal, 0.1 M Tris pH 8 Protein: 40 mg/mL in water |